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. 2021 Jan 13;12(1):90. doi: 10.3390/genes12010090

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Amplification efficiencies and (b) Cq values observed for 105 SARS-CoV-2 positive samples. The values for both parameters for [+] (blue), [−] (green), and [−] fast (red) were almost identical, suggesting that the three tested conditions did not affect the diagnostic capability of the N1 assay. A three-fold decrease in the limit of detection (LOD) was observed for [−] compared with [+] (7.3 versus 23.7 copies/reaction), and a six-fold decrease in the LOD was observed for [−] compared with [−] fast (7.3 versus 44.2 copies/reaction) using synthetic SARS-CoC-2 RNA and probit regression, suggesting that the differences between the tested conditions will be noted only on samples with a very low viral load.