Figure 6.

Pharmacological activation of β₂-ARs increases Mfn1 expression in skeletal muscle. DRP1 (mitochondrial fraction) (a), Fis1 (b), OPA1 (c), Mfn2 (d), and Mfn1 (e) protein expression, and immunoblot representative images (f) in tibialis anterior muscles from mice treated with the non-selective β-AR agonist isoproterenol (ISO—single dose—10 mg/kg, i.p. 30 min), with or without β₂-AR receptor blockade with ICI 118,551 (single dose—10 mg/kg, i.p., 30 min prior to ISO treatment). Enolase and VDAC were used as loading control for total lysate and mitochondrial fraction protein expression, respectively. Data are presented as mean ± SE. * p < 0.05 vs. control and ** p < 0.05 vs. ISO. n = 5/group.