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. 2020 Aug 9;35(9):2134–2148. doi: 10.1093/humrep/deaa148

Figure 4.

Figure 4

CEP120 p.R947 is important to maintain euploid mouse eggs. (A) Oocytes injected with the indicated construct were fixed at metaphase of MI and stained to detect spindles with α-tubulin (green) and DNA (blue). (B) Quality of chromosome alignment was quantified according to the schematic. The distance between the two extreme chromosome bivalents (red dashed line) was normalized to the total spindle area (green dashed line). (C) Quantification of the chromosome alignment in oocytes from (A). Student’s t-test was used to determine significance. (D) Oocytes injected with the indicated construct were matured to metaphase of meiosis II (MII) to perform an in situ chromosome counting assay. Centromeres were detected with anti-centromeric antigen (Crest; red) and DNA was detected with 4′,6-diamidino-2-phenylindole staining (gray). Shown is an eGFP-CEP120:p.R947H-expressing egg with an abnormal number of chromosomes. The extra chromosome is indicated in yellow. (E) Aneuploid eggs at metaphase of MII were counted and expressed as a proportion. Student’s t-test was used to determine significant differences in aneuploid egg frequency between conditions. n, total number of analyzed oocytes. Indicated are mean ± SD (C) or mean ± SEM (E). Student’s t-test was used to determine significance. n.s., not significant; **P < 0.001. Scale bar: 5 μm.