Figure 2.

DOX release from DOX-loaded SLPs (SLPs-DOX) in cultured cells and in vitro. (a) Fluorescence confocal microscopy projection images of malignant melanoma cells treated with SLPs-DOX for 2, 16, and 48 h. The SLPs appear in the green channel, and microtubules are shown in the blue channel. The red nuclear fluorescence is indicative of the presence of released DOX. (b) In vitro drug release profile of the SLPs-DOX particles in physiological conditions (PBS at 37 °C). The results are mean ± SEM of three experimental replicas. (c) Scheme of treatment with SLPs-DOX against metastasis in the lungs.