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. 2020 Jul 7;26(9):689–701. doi: 10.1093/molehr/gaaa051

Figure 4.

Figure 4.

Effect of mouse oocyte vitrification on mitotic arrest deficient 2 localization. (A) Fresh and vitrified/thawed GV oocytes underwent IVM with or without microtubule depolymerizing agent nocodazole (added at GV break down) until Met I (7 h). Vitrified oocytes were also treated during maturation with or without E-64 or Z-VAD-FMK. Met I oocytes were fixed and stained with an anti-MAD2 (mitotic arrest deficient 2) antibody (white), CREST anti-sera (red) to label kinetochores and DAPI to label DNA (blue). Images were obtained using confocal microscopy. (B) Quantification of images in A. The experiment was performed four times. The total number of analyzed oocytes in each group (from all replicates) is specified above each condition within each graph. The scale bar represents 10 μm. The data are expressed as mean ± SEM; one-way ANOVA was used to analyze the data. Values with asterisks vary significantly, ***P < 0.001, ****P < 0.0001. Unless otherwise specified, asterisks above each group denote the statistical analysis of P-value against the control and vitrified groups.