Figure 1.

Schematic diagram for identifying the protein targets of Sub5 using Saccharomyces cerevisiae proteome microarrays. To find the targets, biotinylated Sub5 was probed on Saccharomyces cerevisiae proteome microarrays and later probed with streptavidin-DyLight 650 and anti-His antibody-DyLight 550 for the detection of signal of biotinylated Sub5 that interacted with Saccharomyces cerevisiae proteins, and proteins containing His tag to represent the protein amount of each proteins on Saccharomyces cerevisiae proteome microarrays, respectively. The protein targets of Sub5 were identified using several cutoff parameters and the final list of protein targets of Sub5 were analyzed with bioinformatics tools gene ontology (GO), Protein family (PFAM), Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), and Multiple Expression motifs for Motif Elicitation (MEME).