Figure 1.

Characterization and intracellular localization of the E2-ferritin and ferritin nanoplatform. (a) Schematic representation of the ferritin (above) and E2-ferritin (below) nanoplatform expression cassette. (b) Immunofluorescence assay (IFA) for nanocage intracellular location using anti-His tag antibody (left) and anti-CSFV E2 mAb (right) as primary antibodies followed by Alexa Fluor 555 as secondary antibody using confocal microscopy. (c) Protein expression detected by Western blotting using anti-His tag monoclonal antibody.