Figure 2.

Amplification and purification of E2-ferritin and ferritin nanocarriers. The optimal proliferation conditions for ferritin in (a) and E2-ferritin in (b) np were both 1 MOI in suspension culture by Western blotting analysis. Protein density was measured by Image J software. The protein elution peak for ferritin in (c) and E2-ferritin in (d) were both in 120 mM imidazole by SDS-PAGE. Different concentrations of BSA were used as standard samples. The purified nanocages of ferritin in (e) and E2-ferritin in (f) were verified by immunoblotting using anti-His tag antibody. (For the uncropped blots of Figure 2a,b,e,f, see Supplementary Materials, Figures S2–S5).