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. 2021 Jan 13;9(1):45. doi: 10.3390/vaccines9010045

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The humoral and cellular immunity response detection. (a) Relative IL-4 mRNA fold change detection by RT-qPCR. (b) Relative IFN-γ mRNA fold change detection by RT-qPCR. (c) Lymphocyte proliferation assay for cell viability determination via MTT. All the data in this figure are repeated 4 times. The data presented here were analyzed by two-way ANOVA assay using GraphPad Prism 8.0. *, 0.05 < p < 0.01; **, 0.01 < p < 0.001; ***, p < 0.001.