Table 2.
Immunization procedure and dosages in the ATR evaluation model.
| Cohort (n = 4) |
Dosage | Antigens | Immunization | Challenge | Sacrifice (56 dpi) |
|---|---|---|---|---|---|
| PBS | 1 mL (IM) | Prime vaccination: Day 1; Booster vaccination: Day 21; Intramuscular injection (IM) |
Viral challenge: C-strain (1 dose) Intravenous injection (IV) |
Spleen collection for viral tissue load assay | |
| pfe | 40 μg (IM) | 40 μg purified |
|||
| pE2-fe | 40 μg (IM) | 40 μg purified |
|||
| pE2-fe/Gel02 | 40 μg (IM) | 40 μg purified |
|||
| E2-fe/Gel02 | 40 μg (IM) | 20 μg (unpurified) | |||
| pE2/Gel02 | 40 μg (IM) | 40 μg purified |
|||
| C-strain | 1-dose (IV) | 1-dose |