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. 2021 Jan 13;22(2):753. doi: 10.3390/ijms22020753

Table 2.

Real-time PCR (QPCR) primers for various genes used in this study.

Genes Primers
Forward Sequence (5′–3′) Reverse Sequence (5′–3′)
NFkB GTGACAGGAGACGTGAAGATG TGAAGGTGGATGATTGCTAAGT
E2F1 TCCCTGAGCTGTTCTTCTG CCTCCCTCACTTTCCCAATAAA
STAT3 GAGAAGGACATCAGCGGTAAG CAGTGGAGACACCAGGATATTG
RUNX2 CGGAATGCCTCTGCTGTTAT TGTGAAGACGGTTATGGTCAAG
Snail ACTATGCCGCGCTCTTTC GCTGGAAGGTAAACTCTGGATTA
TWIST1 AGACTCTGGAGCTGGATAACT GCCTGTCTCGCTTTCTCTTT
SOX2 GGACTGAGAGAAAGAAGAGGAGAG CGCCGCCGATGATTGTTATTA
Cyclin D1 GGTTCAACCCACAGCTACTT CAGCGCTATTTCCTACACCTATT
FZD1 AAGACCGAGTGGTGTGTAATG TGGCCATGCTGAAGAAGTAG
GINS1 TCAGGTGGACGAAGTGATTTG CGAAGCAAGCGGTCATACA
MMP9 GAACTTTGACAGCGACAAGAAG CGGCACTGAGGAATGATCTAA
Lgr5 GGGAAACGCTCTGACATACA CTTCTGTGGGTACGTGTCTTAG
OCT4 GGAGGAAGCTGACAACAATGA CTCTCACTCGGTTCTCGATACT
c-Myc AAGCTGAGGCACACAAAGA GCTTGGACAGGTTAGGAGTAAA
EpCAM AGCTGGTGTTATTGCTGTTATTG GCATCTCACCCATCTCCTTTAT
ALDH1 CTTGGAATTTCCCGTTGGTTATG GAGAGCAGTGAGAGGAGTTTG
Nanog GCCTGTAGTCCCAGCTATTTG GGAGTGCAGTGGTGTGATATT
ZEB1 GGCTCCTATAGCTCACACATAAG TGCTGAAAGAGACGGTGAAG
CD44v6 GACAGAATCCCTGCTACCAATAG TCCTTCGTGTGTGGGTAATG