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. 2021 Jan 13;26(2):399. doi: 10.3390/molecules26020399

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence detecting localization behavior of β-catenin in phytochemicals-treated SK-LU-1. SK-LU-1 was pre-treated with GSK3i at 8 μM for 24 h followed by phytochemicals treatments for 24 h. (A) Representative micrographs depicting immunofluorescence staining of β-catenin (orange) counterstained with Hoechst 33342 (blue) to indicate nuclei in phytochemicals-treated SK-LU-1. White circles highlight cells with diminished β-catenin localization in nucleus. Scale bars represent 100 μm. (B) Graph representing percentage of SK-LU-1 with nuclear β-catenin calculated in response to treatment at ¼ IC₅₀, ½ IC₅₀ and IC₅₀ concentrations of respective phytochemicals. The quantification data were derived from a representative immunostaining experiment. Error bars are expressed as mean ± SD from triplicate data in an independent experiment. Statistical significance is expressed as *** p < 0.001; ** p < 0.01.