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Primer sequences and characteristics of the primers used for qRT-PCR.
| Name of Primers | Target Gene | Sequence (5′ > 3′) | Product Size (bp) | Tm |
|---|---|---|---|---|
| CL585C5-F | Tubulin (CL585) | TTCCACTGCTGTTGTTGAGC | 98 | 58 |
| CL585C5-R | AGATGGCCTCATTGTCAACC | |||
| CL844- F | CYP450 (CL844) | CTGGGACTTGTTGTGGACCT | 175 | 58 |
| CL844-R | GGACTTTTCGGTGTGCTTGT | |||
| CL1604C5-F | Vitellogenin (CL1604) | TTTTTGATTGTGACCGACCA | 62 | 58 |
| CL1604C5-R | CAAAAGTCCAGCACCCAGTT |
