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. 2021 Jan 14;10(1):109. doi: 10.3390/antiox10010109

Figure 5.

Figure 5

Effects of NOX, LOX and COX inhibitors on NO and cytokine production in LPS-treated BV2 cells. BV2 cells were grown overnight in low-glucose medium and then transferred to high-glucose medium before treatment with 25 ng/mL LPS for 24 h. Cells were treated with LPS alone or in the presence of (A) 10 µM of the NOX2 inhibitor GSK2795039 or the NOX1/4 inhibitor GKT137831; (B) 10 µM of the 5LOX inhibitor zileuton, the 15LOX inhibitor PD146176 or the COX inhibitor indomethacin. The cell culture supernatants were assayed for NO, IL6 and TNFα production. The results are the average of a minimum of three independent experiments. *** p < 0.001 as compared to LPS alone. ns, not significant.