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. 2021 Jan 14;10(1):153. doi: 10.3390/cells10010153

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Seeding of NPCs by ELYS on anaphase chromosomes was not affected in HGPS. Immunocytochemistry of (a) control (GMO1651c) and (b) HGPS (HGADFN127) fibroblasts using α-ELYS and α-LA antibody, counterstained with DAPI. NPCs cluster in interphase HGPS cells (b), unlike the evenly spread out punctate pattern in interphase control cells (a). Recruitment of ELYS to anaphase chromosomes is not affected in HGPS cells when compared to control. No defect in ELYS localization can be observed in HGPS from prophase to cytokinesis. n ≥ 3.