Figure 8.

Cells with high levels of progerin were senescent. (a) Representative images of cells stained for α-progerin and α-p16/p21 or β-Gal, counterstained with DAPI. Arrows indicate nuclei with a high progerin signal and positive for either high p16 or p21 or beta-Gal. (b) The number of dysmorphic nuclei with strong progerin signal positive for SA-β-Gal and positive for p16 or p21 increased during replicative senescence. (c) The number of dysmorphic nuclei with strong progerin signal remained constant during replicative senescence. At 5% senescence, HGPS cells had a passage number ≤ P18 and at 30% senescence ≥ P21. Values are presented as mean ± SD (n ≥ 3), not significant (ns) p > 0.05, * p < 0.05, ** p < 0.01, (b,c) unpaired t-test, (c) one-way ANOVA with Tukey’s multiple comparison test.