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. 2021 Jan 14;10(1):153. doi: 10.3390/cells10010153

Figure 9.

Figure 9

NPCs clustered in folds of dysmorphic nuclear envelopes and co-localized with progerin/Lamin A aggregates trapped at the nuclear membrane. (a) Representative images of normal control (GMO1651c) and HGPS (HGADFN127) nuclei stained with α-NUP107/POM121/LA/progerin antibodies, counterstained with DAPI. (b) Representative images of dysmorphic control and HGPS nuclei, stained with α-NUP107 and α-LA/C or progerin antibodies, counterstained with DAPI. (c) Representative images of dysmorphic control and HGPS nuclei, stained with α-POM121 and LA antibodies, counterstained with DAPI. Arrows indicate regions with clustered NPCs, outlines indicate zoomed in section of nuclei. All images are taken from z-stack and are provided as X-Z view. Zoom factor: (b) GMO1651c 15x, HGADFN127 25x; (c) GMO1651c 9x, HGADFN127 12x.