Table 1:

Stability of SARS-CoV-2 in donated human milk with and without Holder pasteurization (TCID₅₀/mL)^*

Sample	Unpasteurized (room temperature for 30 min)	Pasteurized (62.5°C for 30 min)
A	2.0 × 105	Undetected
B	6.3 × 104	Undetected
C	6.3 × 105	Undetected
D	6.3 × 106	Undetected
E	6.3 × 105	Undetected
F	2.0 × 105	Undetected
G	6.3 × 105	Undetected
H	6.3 × 105	Undetected
I	6.3 × 105	Undetected
J	6.3 × 105	Undetected
SARS-CoV-2 alone (positive control)	6.3 × 106	6.3 × 103
Mock infection (negative control)	Undetected	Undetected

Note: SARS-CoV-2 = severe acute respiratory syndrome coronavirus 2, TCID₅₀ = 50% of the tissue culture infectivity dose.

^*TCID₅₀/mL calculations defined by duplicate dilution series of indicated samples.