Table 5.

Advantages and Limitations of Different Cell Models for Neutrophil Research

Cell Model	Advantages	Limitations
HL-60	- Most commonly used, many protocols available - Suitable for genetic editing - NETosis described - Easy to culture - Immunosuppressive differentiation described	- Differentiation mechanism not entirely clear - Bacterial killing inefficient - No specific/gelatinase granules
PLB-985*	Same as HL-60	Same as HL-60
NB4	- Clear differentiation mechanism - Easy to culture	- No specific/gelatinase granules - Few protocols available
Kasumi-1	- Suitable for studying early neutrophil differentiation	- Few protocols available - Expensive to culture
Induced pluripotent stem cells	- Closely resembling wildtype neutrophils - Patient-specific cell lines can be created	- Expensive to culture - Protocol time-consuming

Note: *Subline of HL-60 cells.