Figure 2.

Glyphosate induced changes in microtubule stability between the microtubule-enriched fraction (P) and the non-polymer fraction (SN). (A) Western blot analysis against α-tubulin antibody. The quantification of the percentage of polymeric and non-polymeric tubulin with regard to total tubulin (polymer + soluble dimer) indicated that the percentage of polymeric tubulin in the embryos exposed to glyphosate at either 10 or 50 μg/ml was significantly lower than that of the control group (p < 0.05). (B) Western blot analysis against acetylated α-tubulin antibody. The quantification analysis indicated that the percentage of acetylated α-tubulin in the polymeric fraction (P) from the embryos exposed to glyphosate at 50 μg/ml was significantly lower than that of the control group (p < 0.05). Original images of the western blots were provided as supplementary material: (A) Fig_S2A_alphaTub.jpg; (B) Fig_S2B_alphaTubAC.jpg.