REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Experimental models: organisms/strains | ||
w;Pdf(BMRJ)-Gal4/CyO; | (Park et al., 2000, Renn et al., 1999) | n/a |
w;;UAS-Unc5-HA | Barry Dickson, Janelia Farm (Keleman and Dickson, 2001) | n/a |
;;UAS-DbtLONG myc(27MIC)/(TM3) | Jeffrey Price, University of Missouri at Kansas City (Muskus et al., 2007) | n/a |
w1118 | Bloomington Drosophila Stock Center | ID 3605 |
Software and algorithms | ||
R | The R Foundation | RRID:SCR_001905 https://www.r-project.org/ |
DAM FileScan | Trikinetics; (Pfeiffenberger et al., 2010a) | https://www.trikinetics.com/ |
DAM System3 | Trikinetics; (Pfeiffenberger et al., 2010a) | https://www.trikinetics.com/ |
ClockLab (optional) | Actimetrics | RRID:SCR_014309 https://www.actimetrics.com/products/clocklab/ |
MATLAB (optional) | MathWorks | RRID: SCR_001622 https://www.mathworks.com/products/matlab.html |
R code for analyzing and visualizing phases | Dr. Boyan Kostadinov, computational framework developed for this study | The R code developed for this study is available at: https://github.com/bkostadi/STARProtocolsExtendedMethods |
Chemicals, peptides, and recombinant proteins | ||
Bacto Agar | Becton Dickinson Microbiology | Cat#214010 |
Sucrose | Sigma-Aldrich | Cat#S7903; CAS: 57-50-1 |
Paraffin | Fisher Scientific | Cat#T555 |
Other | ||
DAM2 Drosophila activity monitors | Trikinetics; (Pfeiffenberger et al., 2010a) | n/a |
DAM Drosophila environmental monitors | Trikinetics; (Pfeiffenberger et al., 2010a) | n/a |
Fly food | Archon Scientific | Item#W10101 |
Power supply | Trikinetics | #PSIU9 |
Monitor tubes | Trikinetics | #PGT10x100 |
Cable splitter | Trikinetics | #SPLT5 |
Monitor cables | Trikinetics | #CAB2 and #CAB10 |
Brushes for sorting | Flinn Scientific | #: AB1419 |
Benchtop Flowbuddy Complete | Genesee Scientific | Cat#59-122BC |