Figure 2.
Optimization of Incucyte analyzer
(A) HT-1080N cells following initial seeding and after treatment with vehicle control (DMSO) or the lethal proteasome inhibitor bortezomib for 48 h. Scale bar, 100 μm. Cells are imaged using the 10× objective; SYTOX green marks dead cells in the green channel.
(B) Screenshots of Incucyte software showing results of low (top) and high (bottom) edge sensitivity setting on object counting. Scale bars, 20 μm.
(C) Screenshots of Incucyte software showing optimized masking for red, green, and overlap objects. Scale bars, 100 μm.