FIGURE 1.

Human umbilical cord-MSCs-derived EVs prevent HIBD in neonatal mice. HIBD mice were treated with PBS, EVs-free supernatant (EFS) or MSCs-derived EVs (MSCs-EVs) (n = 12/group) to simulate the action on HIBD in neonatus. (A) The morphology or EVs observed using a transmission electron microscope (100 nm). (B) The size and distribution of EVs determined by NanoSight. (C) The protein expression of Alix, TSG101, GM130, and CD63 in EVs assessed by immunoblotting. (D) The distribution of red-labeled (PKH26) EVs in the hippocampus was determined using immunofluorescence (400×). The nuclei were stained blue by DAPI. (E) mNSS in mice following injections of PBS, EFS, or MSCs-EVs. (F) The edema formation in mice after different treatments. (G) The cerebral infarction volume in mice determined by TTC staining. (H) The neuron morphology determined using H&E staining (200×). *p < 0.05 vs. sham-operated mice; #p < 0.05 vs. HIBD mice treated with PBS. Unpaired t-test was applied for the comparisons between two groups and one-way ANOVA followed by Tukey’s multiple comparisons was used for comparisons among three or more groups.