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. 2021 Jan 11;8:579236. doi: 10.3389/fcell.2020.579236

FIGURE 2.

FIGURE 2

Human umbilical cord-MSCs-derived EVs inhibit the OGD-induced neuronal apoptosis in vitro. OGD-exposed neurons were treated with PBS, MSCs-EVs or EFS. (A) The isolated neurons evaluated using immunofluorescence (400×) (NeuN: green; DAPI: blue). (B) The content of LDH released from neurons at 0, 24, and 48 h prior to OGD. (C) Neuronal cell survival rate determined by MTT assay. (D) Neuronal cell-cycle distribution determined using flow cytometry. (E) Neuronal cell apoptosis determined using flow cytometry. (F) The red-labeled (PKH26) EVs in neurons determined using immunofluorescence (500×). *p < 0.05 vs. untreated neurons; #p < 0.05 vs. neurons treated with PBS. Unpaired t-test was applied for the comparisons between two groups and one-way ANOVA followed by Tukey’s multiple comparisons was used for comparisons among more than two groups. Repeated measures ANOVA was conducted to compare data at different time points, followed by Bonferroni’s post hoc test.