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. 2021 Jan 11;8:579236. doi: 10.3389/fcell.2020.579236

FIGURE 3.

FIGURE 3

Extracellular vesicle-derived miR-410 from hUC-MSCs inhibits the OGD-exposed neuronal apoptosis. OGD-exposed neurons treated with equal amounts of purified EVs, together with either 20 μg/μL proteinase K or RNase A supplemented with 0.05% Triton X-100 in panels (A–F). OGD-exposed neurons were introduced with PBS, MSCs-EVs or EFS in panel (G). (A) Gel electrophoresis showing the RNase digested EVs were depleted of RNAs compared with proteinase and control treatment. (B) Silver staining (right) showing that after proteinase treatment, EVs were degraded thoroughly. (C) The intact structure of EVs following enzyme treatment determined by NanoSight. (D) Neuronal cell survival rate determined by MTT assay. (E) Neuronal cell-cycle distribution determined using flow cytometry. (F) Neuronal cell apoptosis determined using flow cytometry. (G) The expression of miR-410 in neurons determined by RT-qPCR following treatment of MSCs-EVs. *p < 0.05 vs. MSCs-EVs or OGD-exposed neurons introduced with MSCs-EVs or neurons. One-way ANOVA followed by Tukey’s multiple comparisons was used for comparisons among more than two groups. Repeated measures ANOVA was conducted to compare data at different time points, followed by Bonferroni’s post hoc test.