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. 2021 Jan 11;8:579236. doi: 10.3389/fcell.2020.579236

FIGURE 7.

FIGURE 7

Extracellular vesicle-derived miR-410 attenuates HIBD via EGR2/Bcl2 upregulation by downregulating HDAC1 in neonatal mice. HIBD mice were injected with PBS, MSCs-EV, lentiviral vectors containing miR-410 antagomir-EV or antagomir-NC-EV (n = 12/group). (A) mNSS in mice after different treatment. (B) The edema formation in mice after different treatments. (C) The cerebral infarction volume in mice determined by TTC staining after different treatments. (D) The neuron morphology in mice determined using H&E staining (200×). (E) The expression of miR-410, HDAC1, EGR2, and Bcl2 determined using RT-qPCR. (F) The expression of cleaved-Caspase-3/pro-Caspase-3, HDAC1, EGR2, BCL2, and acetyl-histone 3 determined by immunoblotting. *p < 0.05 vs. sham-operated mice; #p < 0.05 vs. HIBD mice treated with PBS; &p < 0.05 vs. HIBD mice infected with lentiviral vector containing antagomir-NC-EVs. Unpaired t-test was applied for the comparisons between two groups and one-way ANOVA followed by Tukey’s multiple comparisons was used for comparisons among more than two groups.