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. 2021 Jan 11;8:596363. doi: 10.3389/fcell.2020.596363

FIGURE 3.

FIGURE 3

Upregulation of osteogenic markers and MALAT1 in human renal interstitial fibroblasts (hRIFs) cultured under osteogenic conditions. (A–D) Relative mRNA expression levels of osteogenic markers (Runx2, Osterix, OPN, OCN) were determined by qRT-PCR 0, 1, 4, 7, 10 and 14 days after osteogenic induction. (E) Protein expression levels of osteogenic markers (Runx2, Osterix, OPN, OCN) were determined by WB 0, 1, 4, 7, 10 and 14 days after osteogenic induction. (F) The relative activity of alkaline phosphatase (ALP) was determined by the release of p-nitrophenol 0, 1, 4, 7, 10 and 14 days after osteogenic induction. (G) The relative expression level of MALAT1 was determined by qRT-PCR 0, 1, 4, 7, 10 and 14 days after osteogenic induction. (H–K) Correlation analysis between MALAT1 expression levels and the mRNA expression levels of osteogenic markers after osteogenic induction. GAPDH was used as the internal control. **P < 0.01; ***P < 0.001.