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. 2021 Jan 11;8:596363. doi: 10.3389/fcell.2020.596363

FIGURE 4.

FIGURE 4

MALAT1 promoted osteogenic differentiation of human renal interstitial fibroblasts (hRIFs). (A) Agarose gel electrophoresis showed the PCR products from the 5′ and 3′ RACE of MALAT1 in hRIFs. (B) RNA fluorescence in situ hybridization (FISH) for MALAT1 in isolated hRIFs. (C) Alizarin Red staining in transfected hRIFs 14 days after osteogenic induction (100 ×). (D) The relative MALAT1 expression level was determined by qRT-PCR in transfected hRIFs 7 days after osteogenic induction. (E) Relative alkaline phosphatase (ALP) activity in transfected hRIFs 7 days after osteogenic induction. (F–I) Relative mRNA expression levels of osteogenic markers (Runx2, Osterix, OPN, OCN) were determined by qRT-PCR in transfected hRIFs 7 days after osteogenic induction. (J) Protein expression levels of osteogenic markers (Runx2, Osterix, OPN, OCN) were determined by WB in transfected hRIFs 7 days after osteogenic induction. GAPDH was used as the internal control. *P < 0.05; **P < 0.01; ***P < 0.001.