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. 2021 Jan 11;14:615395. doi: 10.3389/fnins.2020.615395

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Copyright © 2021 Shen, Zhang, Wu, He, Liu, Xu, Yao, He, Chen, Liang, Liu, Jiang, Fang, Du, Zhu, Wu, Wu, Sun, Xu, Fang and Shao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The thalamic neurons receive glutamatergic input from the rACC. (A) Scheme showing the configuration of the experiment. (B) Scheme for specifically labeled glutamatergic neurons in the rACC output to thalamus with mCherry. (C) The representative images of the whole rACC after virus injections. (D) mCherry signals were co-localized with the glutamatergic neuronal marker CaMKIIα in the rACC (scale bars: 20 μm). (E) Representative images of the virus injection site in the rACC after 6 weeks (scale bars: 1 mm in the middle of D, 200 μm in the right of D). (F) Representative images of the anteromedial thalamic nucleus (AM) and ventral anterior thalamic nucleus/ventrolateral thalamic nucleus (VA/VL) with rACC projections (scale bars, 1 mm in the middle of E, 500 μm in the right of E).