Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 11;8:619079. doi: 10.3389/fcell.2020.619079

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Pham, Bink, Stanicek, van Bergen, van Leeuwen, Tran, Matic, Hedin, Wittig, Dimmeler and Boon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Interaction of Aerrie with YBX1 is important for DNA damage signaling. (A) Aerrie-interacting proteins identified by mass spectrometry of RNA-antisense purifications in HUVECs. Identified proteins are plotted against respective IBAQ values. (B–D) HUVECs were treated with gapmeR (gap) targeting Aerrie, siRNA (si) targeting YBX1/2/3 or a respective control. (B) Phosphokinase levels of cell survival-related proteins (n = 4). Red dots are defined as the average level of phosphorylation after silencing knockdown is decreased compared to its respective control. Blue dots are defined as the average level of phosphorylation after silencing knockdown is increased compared to its respective control. (C) Phosphorylation levels of activated proteins in the double-strand break repair pathway were analyzed by western blotting. Phosphorylated ATM at serine 1981, CHK-2 at tyrosine 68, H2AX at serine 139, and P53 at serine 15 were analyzed upon depletion of Aerrie and YBX1 (n > 4). (D) Phosphorylation levels of activated proteins in the single-strand break repair pathway were analyzed by western blotting. Phosphorylated ATR at serine 1989, CHK-1 at serine 317 were analyzed upon depletion of Aerrie and YBX1 (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ns, not statistically significant.