FIGURE 6.

Overexpression of Aerrie improves DNA repair and angiogenic sprouting and is impaired by loss of YBX1/2/3. (A–C) HUVECs were treated with lentivirus (LV) for Aerrie overexpression and siRNA (si) targeting YBX1/2/3 or a respective control. (A) HUVECs with depleted Aerrie or YBX1 are stimulated with Doxorubicin (50 nM) and embedded in low melting agarose stained with SYBR gold and imaged by fluorescence microscopy. Comets were quantified by CometScore. (n > 3, at least 50 comets per experiment) (B) Sprouting assay performed with or without stimulation of Doxorubicin (50 nM). (C) Quantification of the continuous and discontinuous sprouts per condition. Measured by the distance from the tip cell to the stalk cell. (n = 3, ≥10 spheroids per experiment). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not statistically significant.