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. 2021 Jan 14;26(2):410. doi: 10.3390/molecules26020410

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A,B) Cell cytotoxicity of Ptychotis verticillata Essential oil (PV EO) on myeloïd leukemic cell lines and peripheral blood mononuclear cells (PBMCs) from healthy donors. (A): Flow cytometric analysis of cell death with Annexin V-FITC/PI double staining after 24 h treatment of KG1, HL60, and K562 cell lines with increasing concentrations of essential oil. Data were analyzed by Kaluza software 2.1. (B): The quantification of myeloïd leukemic cell lines viability compared to PBMCs from a healthy donor after their treatment with the same concentrations of PV EO (0.01% and 0.02%). Data represent the mean ± SEM of three independent experiments relative to vehicle. The levels of statistical significance were p < 0.001 (***).