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. 2021 Jan 16;22(2):862. doi: 10.3390/ijms22020862

Figure 15.

Figure 15

Immunoblot bar chart showing the expression of the Bad/Bcl-2 ratio w/o the p-ERK inhibitor, PD89059, in MCF-10A (A) and the Bax/Bcl-2 ratio MCF-7 (B) in on-ground control cells, RPM adherent cells, and RPM cell clumps at 24 h. In MCF-10A, columns and bars represent densitometric quantification of the optical density (OD) of a specific protein signal normalized with the OD values of the GAPDH serving as the loading control. In MCF-7, Bax and Bcl-2 were blotted onto the same membrane; thus, Bcl-2 was used as the loading control. Each column represents the mean value ± SD of three independent experiments. In MCF-10A, * p < 0.05 vs. OG; # p < 0.05 vs. RPM by ANOVA followed by Bonferroni post-test. In MCF-7, * p < 0.05, ** p < 0.01 vs. OG e vs. RPM-AD by ANOVA followed by Bonferroni post-test.