Figure 16.

Immunoblot bar chart showing the expression of NF-kB in MCF-10A (A) and in MCF-7 (B) in on-ground control cells, RPM adherent cells, and RPM cell clumps at 24 h w/o the p-ERK inhibitor, PD89059, and w/o the p-AKT inhibitor, LY294002, respectively. Columns and bars represent densitometric quantification of the optical density (OD) of a specific protein signal normalized with the OD values of the GAPDH serving as the loading control. Each column represents the mean value ± SD of three independent experiments. In MCF-10A, ** p < 0.01 vs. OG; ### p < 0.001 vs. RPM by ANOVA followed by Bonferroni post-test. In MCF-7, * p < 0.05 vs. OG by ANOVA followed by Bonferroni post-test.