Table 1.

Roundness, solidity, and fractal dimension (FD) in MCF-7 and MCF-10A at 24 h and 72 h. Mean values ± SD in on-ground control cells, RPM adherent cells, and RPM cell clumps. ** p < 0.01 versus on-ground control cells; *** p < 0.001 vs. on-ground control and RPM adherent cells by ANOVA followed by Bonferroni post-test. (Sample size = 50 cells for each group).

MCF-7 (breast cancer cells, poorly invasive)
	Roundness	± SD		Solidity	± SD		Fractal Demension (FD)	±SD
24 h
on ground	0.646	±0.0151		0.802	±0.101		1.1560	±0.0356
RPM (adherent cells)	0.667	±0.139		0.844	±0.130	***	1.1381	±0.0230
RPM (cell clumps)	0.783	±0.111	***	0.921	±0.034	***	1.1275	±0.1017	***
72 h
on ground	0.619	±0.149		0.759	±0.100		1.1478	±0.1296
RPM (adherent cells)	0.654	±0.143	***	0.773	±0.093		1.1633	±0.0333
RPM (cell clumps)	0.743	±0.130	***	0.897	±0.063	***	1.1385	±0.0248
MCF-10A (non tumorigenic mammary)
	Roundness	±SD		Solidity	±SD		Fractal Demension (FD)	±SD
24 h
on ground	0.709	0.154		0.788	0.259		1.344	0.088
RPM	0.713	0.135		0.821	0.215		1.371	0.096	**
72 h
on ground	0.726	0.131		0.856	0.132		1.391	0.092
RPM	0.711	0.125		0.860	0.090		1.443	0.081	***