Figure 1.

Schematic illustration showing the procedure of scRNA-seq in gonadal tissues. Reproductive tissues are isolated and enzymatically dissociated. Highly pure single cell populations are obtained by conventional cell sorting methods such as fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS). Uniquely barcoded beads are required for microfluid-based scRNA-seq. Technically, one cell is interacted with a bead, and subsequently the cells are subjected to cell lysis for the preparation of mRNAs. The isolated mRNAs are used for reverse transcription. Finally, scRNA-seq libraries containing bead-specific oligo sequences and unique molecular identifier (UMI) are generated.