Table 1.
Evaluation of hepatotoxicity and safety of LEPE in serum.
| Sham 2 | Vehicle | LEPE100 | LEPE200 | E2 | |
|---|---|---|---|---|---|
| AST (IU/L) 1 | 107.50 ± 10.14 | 108.20 ± 7.98 | 130.40 ± 4.90 | 114.90 ± 6.43 | 83.05 ± 17.11 |
| ALT (IU/L) | 33.42 ± 2.33 | 41.70 ± 5.10 | 39.35 ± 5.53 | 37.75 ± 3.70 | 41.03 ± 4.01 |
| Estradiol (pg/mL) | 440.90 ± 22.85 ab | 408.60 ± 8.79 b | 456.10 ± 14.11 ab | 460.30 ± 13.93 ab | 479.70 ± 11.92 a |
| FSH (ng/mL) | 1.88 ± 0.36 | 1.09 ± 0.26 | 0.66 ± 0.05 | 1.63 ± 0.29 | 1.66 ± 0.29 |
| Uterine weight (mg) | 602.60 ± 42.79 a | 118.10 ± 7.50 c | 117.30 ± 6.94 c | 118.00 ± 3.53 c | 303.60 ± 14.26 b |
1 AST, aspartate transaminase; ALT, alanine aminotransferase; FSH, follicle-stimulating hormone. 2 Sham, sham-operated rats; Vehicle, ovariectomized (OVX) rats treated with vehicle; LEPE100, OVX rats treated with Leonurus japonicus Houtt, Eclipta prostrata L., and Pueraria lobata Ohwi (LEPE) 100 mg/kg bw; LEPE200, OVX rats treated with LEPE 200 mg/kg bw; E2, positive control, OVX rats injected with estradiol 3.0 µg/kg bw. Data are shown as the mean ± SEM (n = 12). a–c Significant differences were analyzed by one-way ANOVA with Tukey’s multiple comparisons between groups (p < 0.05).