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Construction and characterization of rBCG-MyHCα. (a) Oligonucleotides that encode a MyHCα CD4 epitope were cloned into an XhoI site in the coding region of the Ag85B gene from Mycobacterium kansasii cloned into pSO246 to be fused with the gene, and the plasmid was introduced into BCG by electroporation, giving rise to rBCG-MyHCα. (b) Western blot analysis of recombinant Ag85 protein or M. kansasii, cell lysates of BCG and rBCG-MyHCα, and culture supernatants of BCG and rBCG-MyHCα.
