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Culture supernatant of rBCG-MyHCα-infected DCs promoted Th1 and Th17 differentiation. Naïve CD4+ T cells were cultured in the supernatant of rBCG-MyHCα-infected BMDCs for 48 h. The gene expression of Tbx21 and Rorc were assessed by qRT-PCR and cytokine concentrations in the culture supernatant were assessed by ELISA. The values are expressed as means of pentuiplicate wells. * p < 0.05 vs. MOI 0 by one-way ANOVA with Tukey’s post hoc test.
