Figure 2.
Bacterial recovery from tadpoles 3, 14, and 40 days after intraperitoneal (IP) inoculation of either R or S Mab. (A) Timeline and (B) two-by-two comparison. Tadpoles were IP-injected with 5 × 105 CFU in a 10 μL volume. Spleen, liver, peritoneal leukocytes (PLs), and lungs were collected, suspended in 500 µL of aPBS, lysed by bead beating, and plated on agar media. The bacterial abundance was determined by the number of CFUs/tissue. **p < 0.005, ***p < 0.0005, and ****p < 0.0001 (two-way ANOVA followed by post hoc analysis using Tukey’s multiple comparisons test).