Figure 4.
Bacterial recovery from tadpoles 3 and 14 days after intracardiac (IC) inoculation of either R or S Mab. (A) Comparison of R and S Mab CFUs for different tissues at 3 and 14 dpi. (B) CPU comparison between 3 and 14 dpi for R and S Mab. Tadpoles were IC-injected with 5 × 105 CFU in a 10 μL volume. Spleen, liver, PLs, and lungs were collected, suspended in 500 µL of aPBS, lysed by bead beating, and plated on agar media. The bacterial abundance was determined by the number of CFUs/tissue. * p < 0.05, ** p < 0.005, and **** p < 0.0001 (two-way ANOVA followed by post hoc analysis using Tukey’s multiple comparisons test).