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. 2021 Jan 16;13(2):319. doi: 10.3390/cancers13020319

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RT-qPCR analyses of the effects of UNC treatment on purified UCB-CD34⁺ cell precursors undergoing differentiation after 6 days of culture in the absence or in the presence of UNC 1999. Results are presented as Log2 fold change versus CD34⁺ cell control T0′ and 6 days (dd), respectively of relative quantification analysis efficiency method based on gene target compared to the 18S rRNA and ACTB housekeeping genes. Values are the mean ± SEM of RT-qPCR analyses on three different biological replicates. (*** p < 0.001).