Effect of environmental conditions on growing pigs for measures of complete fatty acid oxidation (FAO-CO2) (A), acid soluble metabolites (incomplete oxidation) (FAO-ASM) (B), total-FAO (FAO-CO2 + FAO-ASM) (C), metabolic flexibility (pyruvate oxidation:pyruvate oxidation +100 µM palmitic acid) (D), pyruvate oxidation (E), and leucine oxidation (F). Pigs were acclimated under thermoneutral conditions (14 h light/10 h dark, 21.6 ± 0.8 °C, with humidity 46–64%) for five days prior to treatment (Pre). Pigs were then randomly assigned (n = 8 per group) to thermal neutral (TN, 22.0 ± 0.4 °C, with humidity 43–63%) or heat stress (HS, 33.6 ± 0.5 °C, with humidity 22–40%) treatment groups for five days (Post). Means without a shared letter are significantly different, p < 0.05.