Figure 3.

In vitro study of hDPSCs on the scaffolds. (a) Fluorescence microscopy image of the hDPSCs attached on the scaffolds on day 3. Both O₂ plasma and NaOH treatments enhanced cell attachment of the scaffolds. (b) Cell proliferation on days 1, 3, and 7 using a WST-1 assay (n = 7, ANOVA, Duncan’s multiple range test, p < 0.05). At day 7, the NaOH-treated scaffolds exhibited significantly enhanced cell proliferation. Same letters indicate that there is no significant difference between samples. (c) Measurement of protein adsorption on the scaffolds after 24 h of incubation with 1% bovine serum albumin (BSA) solution (n = 5, ANOVA, Duncan’s multiple range test, p < 0.05). O₂ plasma treatment greatly promoted protein adsorption ability of the scaffolds. Same letters indicate that there is no significant difference between samples.