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. 2021 Jan 17;11(1):64. doi: 10.3390/life11010064

Figure 4.

Figure 4

Gradient purification and detection of HBcHEV ORF2 551–607 virus-like particles (VLPs). (a) SDS-PAGE analysis of fractionated samples from the sucrose cushions, the gel was stained with InstantBlue; (b) Western blot profiles showing that the VLPs were successfully detected in the 70% sucrose and interface fractions using HBcAg monoclonal antibody 10E11. M, marker; 1, pEAQ-HT; 2, crude extract; 3, supernatant; 4, pellet; 5, ultracentrifugation supernatant; 6, 25% sucrose gradient fraction; 7, interface and 70% sucrose gradient fraction; 8, positive control recombinant HBcAg (Jena BioSsience, Germany); (c) SDS-PAGE of fractioned VLPs from the Nycodenz gradients. M, marker; 1, fractioned HBcAg VLPs; 2, fractined HBcHEV ORF2 551–607 VLPs. SeeBlue Plus2 Pre-stained Protein Standard was used as a molecular marker.