Table 2.

Analytical methods used to determine the quality parameters of acid oils and fatty acid distillates.

Analytical Method	Main Reference 1
Sample preparation	Varona et al., under review [19]
M	AOCS Official Method Ca 2d-25 [21]
I	ISO 663:2017 [22]
U	AOCS Official Method Ca 6b-53 [23]
FFA-AC	ISO 660:2009 [24]
FA composition	Guardiola et al. [25]
POL, TAG, DAG, MAG and FFA-SE	IUPAC, standard method 2508 [26]
T and T3	Aleman et al. [27]
Dietary energy, calculated	Wiseman et al. [28]

¹ All protocols are detailed in [19]. Abbreviations: M, moisture and volatile matter; I, insoluble impurities; U, unsaponifiable matter; FFA-AC, acidity; FA, fatty acid; POL, polymeric compounds; TAG, triacylglycerols; DAG, diacylglcyerols; MAG, monoacylglycerols; FFA-SE, free fatty acids fraction; T, tocopherols; T3, tocotrienols. Note: the content of FFA was determined by titration (FFA-AC) and by size molecular exclusion chromatography (FFA-SE; IUPAC, standard method 2508)