Table 1.
Peptide sequences of Mag2 and tested peptides 1–9, antimicrobial activity against gram-positive and gram-negative bacteria, and hemolytic activity against red blood cells (RBCs). X indicates the replacement of amino acid residue with Aib residues.
| Peptide | Sequence | MIC (µM) | Hemolysis (µM) | |||
|---|---|---|---|---|---|---|
| Gram Positive | Gram Negative | |||||
| S.a. | E.c. (DH5α) | P.a. | MDRP | |||
| Mag2 | H-GIGKFLHSAKKFGKAFVGEIMNS-NH2 | 100 | 3.125 | 12.5 | 12.5 | >100 |
| 1 | H-GIKKFLKSXKKFVKXFK-NH2 | 12.5 | 3.125 | 3.125 | 3.125 | 50 |
| 2 | H-S5IKKS5LKSAKKFVKAFK-NH2 | 3.125 | 1.56 | 1.56 | 1.56 | 50 |
| 3 | H-GIKKS5LKSS5KKFVKAFK-NH2 | 3.125 | 1.56 | 3.125 | 0.78 | 6.25 |
| 4 | H-GIKKFLKS5AKKS5VKAFK-NH2 | 6.25 | 1.56 | 1.56 | 0.78 | 3.125 |
| 5 | H-GIKKFLKSS5KKFS5KAFK-NH2 | 12.5 | 1.56 | 6.25 | 3.125 | 1.56 |
| 6 | H-GIKKFLKSAKKS5VKAS5K-NH2 | 3.125 | 1.56 | 1.56 | 0.78 | 3.125 |
| 7 | H-GIKKR8LKSAKKS5VKAFK-NH2 | 25 | 3.125 | 3.125 | 1.56 | 6.25 |
| 8 | H-GIKKFLKR8AKKFVKS5FK-NH2 | >50 | 6.25 | >50 | 3.125 | 0.19 |
| 9 | H-GIKKFLKSR8KKFVKAS5K-NH2 | 6.25 | 3.125 | 6.25 | 3.125 | 3.125 |
S5: (S)-4-pentenyl alanine; R8, (R)-7-octenyl alanine; MIC, minimal inhibitory concentration; S.a., Staphylococcus aureus; E.C., Escherichia coli DH5α; P.a., Pseudomonas aeruginosa; MDRP, multiple-drug resistant P. aeruginosa.