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Temporal expression patterns of HBcAg-wDIII plant leaves. Total protein from N. benthamiana leaves infiltrated with HBcAg-wDIII construct was extracted on 4–10 DPI and analyzed by an ELISA with the E16 mAb, which recognizes a conformational epitope on wDIII as the capture antibody, and detected with a polyclonal anti-wDIII antibody. The means ± SD of samples from three independent experiments are presented.
