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Western blot analysis of HBcAg-wDIII expression in N. benthamiana. HBcAg-wDIII was extracted from leaves and separated on SDS-PAGE gels and transferred onto PVDF membranes. wDIII-specific E16 mAb was used to detect HBcAg-wDIII. lane 1: negative control leaf proteins extracted from un-infiltrated leaves; lanes 2: sample collected 6 DPI from leaves agroinfiltrated with HBcAg-wDIII construct; lane 3: E. coli-produced wDIII as a positive control.
