Figure 7.

Competitive binding between the E16 mAb and antibodies in anti-HBcAg-wDIII serum to wDIII displayed on the yeast cell surface. wDIII-displaying yeast cells were pre-incubated with PBS (A) or week-11 pooled sera (1:1000 dilution) from mice either injected with PBS (B) or HBcAg-wDIII VLP (C). The E16 mAb was then incubated with yeast cells. The specific binding between E16 and yeast-displayed wDIII was measured by staining with an Alexa Fluor 488-conjugated goat anti-human secondary antibody and processing by flow cytometry.